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t1-405(T144E,T150E)
(Plasmid #166130)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 166130 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTrcHis C
  • Backbone manufacturer
    Thermo Fisher Scientific, Carlsbad, CA
  • Total vector size (bp) 5645
  • Modifications to backbone
    includes an N-terminal hexahistidine tag (MGGSHHHHHHGMASMTGGQQMGRDLYDDDD- KDRWIRPRA) before the insert.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use BL21-Star (DE3), or other suitable strain for expression. 37C for 5h growing after induction. At lower temperature can be O/N. IPTG as the inducing agent
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HuT1head1-405(T144E,T150E)
  • Alt name
    talin-1 head
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1218
  • Mutation
    residues 1-405, mutations T144E and T150E
  • Entrez Gene
    TLN1 (a.k.a. ILWEQ, TLN, talin-1)
  • Tag / Fusion Protein
    • His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    t1-405(T144E,T150E) was a gift from Vesa Hytönen (Addgene plasmid # 166130 ; http://n2t.net/addgene:166130 ; RRID:Addgene_166130)

  • For your References section:

    The F1 loop of the talin head domain acts as a gatekeeper in integrin activation and clustering. Kukkurainen S, Azizi L, Zhang P, Jacquier MC, Baikoghli M, von Essen M, Tuukkanen A, Laitaoja M, Liu X, Rahikainen R, Orlowski A, Janis J, Maatta JAE, Varjosalo M, Vattulainen I, Rog T, Svergun D, Cheng RH, Wu J, Hytonen VP, Wehrle-Haller B. J Cell Sci. 2020 Oct 12;133(19). pii: 133/19/jcs239202. doi: 10.1242/jcs.239202. 10.1242/jcs.239202 PubMed 33046605
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