t1-405(del37/GAG)
(Plasmid #166129)

• Purpose: Expression of human talin-1 head (aa1-405) in E. coli. Contains 37 amino acid deletion in F1-loop, replaced with Gly-Ala-Gly. N-terminal His6, Xpress-epitopeDLYDDDDK) and enterokinase cleavage site.
• Depositing Lab: Vesa Hytönen
• Publication: Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 133/19/jcs239202. doi: 10.1242/jcs.239202.

Backbone

• Vector backbone: pTrcHis C
• Backbone manufacturer: Thermo Fisher Scientific, Carlsbad, CA
• Total vector size (bp): 5543
• Modifications to backbone: includes an N-terminal hexahistidine tag (MGGSHHHHHHGMASMTGGQQMGRDLYDDDD- KDRWIRPRA) before the insert.
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Use BL21-Star (DE3), or other suitable strain for expression. 37C for 5h growing after induction. At lower temperature can be O/N. IPTG as the inducing agent
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: T1head1-405(del37/GAG)
• Alt name: talin-1 head
• Species: H. sapiens (human)
• Insert Size (bp): 1116
• Mutation: aa1-405, with 37 aa deletion in the F1-loop which is replaced with Gly-Ala-Gly
• Entrez Gene: TLN1 (a.k.a. ILWEQ, TLN, talin-1)
• Tag / Fusion Protein:
  • His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site (N terminal on insert)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry