pEGFP_S1750F-talin1 (1-2541)
(Plasmid
#166120)
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PurposeFor expression of full-length mouse talin-1 carrying mutation S1750F in mammalian cells. Contains C-terminal EGFP tag.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 166120 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4734
- Total vector size (bp) 12357
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Vector typeMammalian Expression ; CMV promoter
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsFor MidiPrep according to the instructions from the kit.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameS1750F-Talin-1
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Alt nameS1750F-TLN1
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SpeciesM. musculus (mouse)
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Insert Size (bp)7623
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MutationS1750F
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Entrez GeneTln1 (a.k.a. Tln)
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Tag
/ Fusion Protein
- EGFP (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP_S1750F-talin1 (1-2541) was a gift from Vesa Hytönen (Addgene plasmid # 166120 ; http://n2t.net/addgene:166120 ; RRID:Addgene_166120) -
For your References section:
Cancer associated talin point mutations disorganise cell adhesion and migration. Azizi L, Cowell AR, Mykuliak VV, Goult BT, Turkki P, Hytonen VP. Sci Rep. 2021 Jan 11;11(1):347. doi: 10.1038/s41598-020-77911-4. 10.1038/s41598-020-77911-4 PubMed 33431906