HCP4
(Plasmid #166106)

• Purpose: This plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the center of Cyr1 and the other targets a non-coding region on chromosome X (location X1)
• Depositing Lab: Matthias Heinemann
• Publication: Heinemann Lab Plasmids (unpublished)

Backbone

• Vector backbone: pYTK102
• Vector type: Yeast Expression, CRISPR
• Selectable markers: Nourseothricin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cyr1Del-sgRNA/X1-sgRNA
• gRNA/shRNA sequence: Cyr1: TTGGTCATAGAAAGCGACCG, X1 locus: GCTACGGCCGAAGCCAATTC
• Species: S. cerevisiae (budding yeast)
• Entrez Gene: CYR1 (a.k.a. YJL005W, CDC35, FIL1, HSR1, SRA4, TSM0185)

Cloning Information

• Cloning method: Gateway Cloning

Resource Information

• Supplemental Documents:
  • HCP4 annotated map

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry