HCP3
(Plasmid #166105)

• Purpose: This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination
• Depositing Lab: Matthias Heinemann
• Publication: Heinemann Lab Plasmids (unpublished)

Backbone

• Vector backbone: pYTK103
• Vector type: Yeast Expression, CRISPR
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Msn2-sgRNA#27
• gRNA/shRNA sequence: ATCAAGACTCATAAAAAACA
• Species: S. cerevisiae (budding yeast)
• Entrez Gene: MSN2 (a.k.a. YMR037C)

Cloning Information

• Cloning method: Gateway Cloning

Resource Information

• Supplemental Documents:
  • HCP3 annotated map

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry