pPH3
(Plasmid
#166098)
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PurposeGal inducible expression of D10A-Cas9 nickase
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 166098 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep414
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Backbone manufacturerAddgene 43804
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Vector typeYeast Expression, CRISPR
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Selectable markersTRP1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCas9-D10A
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SpeciesSynthetic; S pyogenes Cas9
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MutationspCas9-D10A
- Promoter Gal-inducible
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPH3 was a gift from Rebecca Burgess (Addgene plasmid # 166098 ; http://n2t.net/addgene:166098 ; RRID:Addgene_166098)