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Addgene

pITI1
(Plasmid #166094)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 166094 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p415
  • Backbone manufacturer
    Addgene 43804
  • Vector type
    Yeast Expression, CRISPR
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCas9-FokI
  • Species
    Synthetic; S pyogenes Cas9
  • Mutation
    spCas9-D10A, H840A
  • Promoter Gal-inducible

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    FokI-dCas9 from Addgene plasmid 52970 amplified and cloned into Addgene plasmid 43804 using homology

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pITI1 was a gift from Rebecca Burgess (Addgene plasmid # 166094 ; http://n2t.net/addgene:166094 ; RRID:Addgene_166094)
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