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Addgene

c-myc promoter (Frag B)
(Plasmid #16606)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16606 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBV-Luc
  • Backbone manufacturer
    Vogelstein Lab
  • Backbone size w/o insert (bp) 4867
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    c-myc promoter (Frag B)
  • Alt name
    MYC
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    453
  • Mutation
    FragB (KpnI->AccI). The KpnI site is at -1194 and the AccI site is at -741 on the promoter.
  • Entrez Gene
    MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See Author's Map and article for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    c-myc promoter (Frag B) was a gift from Bert Vogelstein (Addgene plasmid # 16606 ; http://n2t.net/addgene:16606 ; RRID:Addgene_16606)

  • For your References section:

    Identification of c-MYC as a target of the APC pathway. He TC, Sparks AB, Rago C, Hermeking H, Zawel L, da Costa LT, Morin PJ, Vogelstein B, Kinzler KW. Science. 1998 Sep 4. 281(5382):1509-12. 10.1126/science.281.5382.1509 PubMed 9727977
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