NK803
(Plasmid
#166057)
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PurposePuromycin resistance and LifeAct-mCherry expression for Salpingoeca rosetta
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 166057 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneNK802
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Vector typeS. rosetta expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert namePac puromycin resistance gene
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SpeciesSynthetic
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Insert Size (bp)600
Gene/Insert 2
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Gene/Insert nameLifeAct-mCherry
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SpeciesSynthetic
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
NK803 was a gift from Nicole King (Addgene plasmid # 166057 ; http://n2t.net/addgene:166057 ; RRID:Addgene_166057) -
For your References section:
A flagellate-to-amoeboid switch in the closest living relatives of animals. Brunet T, Albert M, Roman W, Coyle MC, Spitzer DC, King N. Elife. 2021 Jan 15;10. pii: 61037. doi: 10.7554/eLife.61037. 10.7554/eLife.61037 PubMed 33448265