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Addgene

AH6114
(Bacterial strain #166038)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Bacterial Strain 166038 Bacteria in agar stab 1 $85
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Backbone

  • Vector backbone
    N/A
  • Selectable markers
    LacZ (repressed)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Spectinomycin, 100 & 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    This is a strain
  • Growth instructions
    Please grow using the following selection: Spectinomycin (20ug/ml) and Ampicillin (100ug/ml)
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    N/A

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Genotype: MG1655 rph+ ΔlacIZYA ΔλattB::lacOid_λattB_PlacUV5.lacO2_LacZ (pIT3-SH-pLacI-LacI reverse)φHK022attB

This strain contains the pInt-ts lambda helper plasmid (Addgene #66076) necessary for the genome integration of pLOM2-500 plasmid (Addgene #164868) or its derivative. This strain expresses lac repressor.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AH6114 was a gift from Keith Shearwin (Addgene plasmid # 166038)

  • For your References section:

    The loopometer: a quantitative in vivo assay for DNA-looping proteins. Hao N, Sullivan AE, Shearwin KE, Dodd IB. Nucleic Acids Res. 2021 Jan 28. pii: 6123373. doi: 10.1093/nar/gkaa1284. 10.1093/nar/gkaa1284 PubMed 33511418
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