AH6112
(Bacterial strain
#166036)
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PurposeLoopometer recipient strain: lacI- Oid+ control
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Depositing Lab
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Sequence Information
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Full plasmid sequence is not available for this item.
No maps are available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Bacterial Strain | 166036 | Bacteria in agar stab | 1 | $85 | |
Backbone
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Vector backboneN/A
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Selectable markersLacZ
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Spectinomycin, 100 & 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)This is a strain
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Growth instructionsPlease grow using the following selection: Spectinomycin (20ug/ml) and Ampicillin (100ug/ml)
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameN/A
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Genotype: MG1655 rph+ ΔlacIZYA ΔλattB::lacOid_λattB_PlacUV5.lacO2_LacZ (pIT3-SH)φHK022attB
This strain contains the pInt-ts lambda helper plasmid (Addgene #66076) necessary for the genome integration of pLOM2-500 plasmid (Addgene #164868) or its derivative. This strain does not express lac repressor.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AH6112 was a gift from Keith Shearwin (Addgene plasmid # 166036) -
For your References section:
The loopometer: a quantitative in vivo assay for DNA-looping proteins. Hao N, Sullivan AE, Shearwin KE, Dodd IB. Nucleic Acids Res. 2021 Jan 28. pii: 6123373. doi: 10.1093/nar/gkaa1284. 10.1093/nar/gkaa1284 PubMed 33511418