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Addgene

pRPN265NB-enh-MNase
(Plasmid #166035)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 166035 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-2T
  • Backbone manufacturer
    GE Healthcare Life Sciences
  • Backbone size w/o insert (bp) 4300
  • Total vector size (bp) 5811
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Glutathione-S-Transferase/enhancer/MNase
  • Species
    S. japonia/camelidae/Staphylococcus aureus
  • Insert Size (bp)
    1512
  • GenBank ID
    P00644
  • Promoter lac promoter / tac promoter
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer pGEX 5`
  • 3′ sequencing primer pGEX 3`
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRPN265NB-enh-MNase was a gift from Marc Timmers (Addgene plasmid # 166035 ; http://n2t.net/addgene:166035 ; RRID:Addgene_166035)

  • For your References section:

    Integrating quantitative proteomics with accurate genome profiling of transcription factors by greenCUT&RUN. Nizamuddin S, Koidl S, Bhuiyan T, Werner TV, Biniossek ML, Bonvin AMJJ, Lassmann S, Timmers H. Nucleic Acids Res. 2021 May 21;49(9):e49. doi: 10.1093/nar/gkab038. 10.1093/nar/gkab038 PubMed 33524153

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