pRS405red-phlTA2-1E
(Plasmid
#166029)
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PurposeExpresses phlTA(2-1E) mutant
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 166029 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRS405red
- Total vector size (bp) 7670
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Vector typeYeast Expression, Synthetic Biology
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namephlF
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SpeciesPseudomonas protegens
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MutationP5S, S6P, K86T, F109L, Q117R, E143K, codon-optimized for expression in yeast
- Promoter pPGK1 mutant
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Tags
/ Fusion Proteins
- three copies of VP16 (VP48) (C terminal on insert)
- Nuclear localization signal (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7, M13/pUC Forward
- 3′ sequencing primer M13/pUC Reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRS405red-phlTA2-1E was a gift from Jun Ishii (Addgene plasmid # 166029 ; http://n2t.net/addgene:166029 ; RRID:Addgene_166029) -
For your References section:
Robust and flexible platform for directed evolution of yeast genetic switches. Tominaga M, Nozaki K, Umeno D, Ishii J, Kondo A. Nat Commun. 2021 Mar 23;12(1):1846. doi: 10.1038/s41467-021-22134-y. 10.1038/s41467-021-22134-y PubMed 33758180
