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Addgene

pGK401red-hENT1
(Plasmid #166028)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 166028 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGK401red
  • Total vector size (bp) 7330
  • Vector type
    Yeast Expression, Synthetic Biology
  • Selectable markers
    MET15

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human equilibrative nucleoside transporter 1
  • Alt name
    hENT1
  • Species
    H. sapiens (human)
  • Entrez Gene
    SLC29A1 (a.k.a. AUG, ENT1, hENT1)
  • Promoter pPGK1

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer n/a
  • 3′ sequencing primer M13/pUC Reverse
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Nick Rhind (hENT1)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

hENT1 gene was cloned from addgene plasmid pFS181 (#12536, Depositor: Nick Rhind)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGK401red-hENT1 was a gift from Jun Ishii (Addgene plasmid # 166028 ; http://n2t.net/addgene:166028 ; RRID:Addgene_166028)

  • For your References section:

    Robust and flexible platform for directed evolution of yeast genetic switches. Tominaga M, Nozaki K, Umeno D, Ishii J, Kondo A. Nat Commun. 2021 Mar 23;12(1):1846. doi: 10.1038/s41467-021-22134-y. 10.1038/s41467-021-22134-y PubMed 33758180
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