pAAV-CaMKII-GCaMP6f-4x128-mAGNET
(Plasmid #166023)

• Purpose: Expresses GCaMP6f in CaMKII+ cells with low miRNA-128 expression
• Depositing Lab: Xue Han
• Publication: Keaveney et al J Neurosci. 2020 Dec 9;40(50):9576-9588. doi: 10.1523/JNEUROSCI.2570-19.2020. Epub 2020 Nov 6.

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• Serotype: Select serotype for details See details about
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Backbone

• Vector backbone: AAV
• Total vector size (bp): 6878
• Vector type: Mammalian Expression, Mouse Targeting, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: GCaMP6f with 4 miR-128 binding sites
• Species: M. musculus (mouse)
• Insert Size (bp): 1482
• GenBank ID:
• Tags / Fusion Proteins:
  • None
  • None
  • None

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that 3' ITR is not annotated on Addgene's plasmid map, however the plasmid can still be packaged and functions as expected.