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Addgene

pAAV-CaMKII-GCaMP6f-4x128-mAGNET
(Plasmid #166023)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 166023 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    AAV
  • Total vector size (bp) 6878
  • Vector type
    Mammalian Expression, Mouse Targeting, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GCaMP6f with 4 miR-128 binding sites
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1482
  • GenBank ID
  • Tags / Fusion Proteins
    • None
    • None
    • None

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that 3' ITR is not annotated on Addgene's plasmid map, however the plasmid can still be packaged and functions as expected.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-CaMKII-GCaMP6f-4x128-mAGNET was a gift from Xue Han (Addgene plasmid # 166023 ; http://n2t.net/addgene:166023 ; RRID:Addgene_166023)

  • For your References section:

    CaMKIIalpha-Positive Interneurons Identified via a microRNA-Based Viral Gene Targeting Strategy. Keaveney MK, Rahsepar B, Tseng HA, Fernandez FR, Mount RA, Ta T, White JA, Berg J, Han X. J Neurosci. 2020 Dec 9;40(50):9576-9588. doi: 10.1523/JNEUROSCI.2570-19.2020. Epub 2020 Nov 6. 10.1523/JNEUROSCI.2570-19.2020 PubMed 33158963
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