pAAV-CaMKII-GCaMP6f
(Plasmid
#166022)
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PurposeExpresses GCaMP6f in CaMKII+ cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 166022 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneAAV
- Total vector size (bp) 6767
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Vector typeMammalian Expression, Mouse Targeting, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGCaMP6f
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SpeciesM. musculus (mouse)
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Insert Size (bp)1353
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GenBank ID
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Tag
/ Fusion Protein
- None
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that 3' ITR is not annotated on Addgene's plasmid map, however the plasmid can still be packaged and functions as expected.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-CaMKII-GCaMP6f was a gift from Xue Han (Addgene plasmid # 166022 ; http://n2t.net/addgene:166022 ; RRID:Addgene_166022) -
For your References section:
CaMKIIalpha-Positive Interneurons Identified via a microRNA-Based Viral Gene Targeting Strategy. Keaveney MK, Rahsepar B, Tseng HA, Fernandez FR, Mount RA, Ta T, White JA, Berg J, Han X. J Neurosci. 2020 Dec 9;40(50):9576-9588. doi: 10.1523/JNEUROSCI.2570-19.2020. Epub 2020 Nov 6. 10.1523/JNEUROSCI.2570-19.2020 PubMed 33158963