R619-M87-303: CMV51p> SARS-CoV S-2P-T4f-3C-His8-Strep2x2
(Plasmid
#166012)
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Purposemammalian expression of SARS-CoV soluble spike trimer protein
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 166012 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDest-303
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSARS-CoV S (spike)
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SpeciesOther
- Promoter CMV51
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Tag
/ Fusion Protein
- 3C-His8-Strep2x2 (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
DNA sequence optimized for mammalian expression
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
R619-M87-303: CMV51p> SARS-CoV S-2P-T4f-3C-His8-Strep2x2 was a gift from Dominic Esposito (Addgene plasmid # 166012 ; http://n2t.net/addgene:166012 ; RRID:Addgene_166012) -
For your References section:
Optimizing high-yield production of SARS-CoV-2 soluble spike trimers for serology assays. Esposito D, Mehalko J, Drew M, Snead K, Wall V, Taylor T, Frank P, Denson JP, Hong M, Gulten G, Sadtler K, Messing S, Gillette W. Protein Expr Purif. 2020 Jun 4;174:105686. doi: 10.1016/j.pep.2020.105686. 10.1016/j.pep.2020.105686 PubMed 32504802