pET19b-rShHTL7 cPGFP(D165)
(Plasmid
#166003)
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PurposeExpression of coding sequence in Escherichia coli
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 166003 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET19b
- Backbone size w/o insert (bp) 5717
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namerShHTL7 cPGFP(D165)
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SpeciesSynthetic
- Promoter T7 promoter
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET19b-rShHTL7 cPGFP(D165) was a gift from Claudia Vickers (Addgene plasmid # 166003 ; http://n2t.net/addgene:166003 ; RRID:Addgene_166003) -
For your References section:
Rational Design of Novel Fluorescent Enzyme Biosensors for Direct Detection of Strigolactones. Chesterfield RJ, Whitfield JH, Pouvreau B, Cao D, Alexandrov K, Beveridge CA, Vickers CE. ACS Synth Biol. 2020 Aug 21;9(8):2107-2118. doi: 10.1021/acssynbio.0c00192. Epub 2020 Aug 11. 10.1021/acssynbio.0c00192 PubMed 32786922