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Addgene

pET19b-DAD2 cPGFP(N180)
(Plasmid #166000)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 166000 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET19b
  • Backbone size w/o insert (bp) 5717
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DAD2 cPGFP(N180)
  • Species
    Synthetic
  • Promoter T7 promoter

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET19b-DAD2 cPGFP(N180) was a gift from Claudia Vickers (Addgene plasmid # 166000 ; http://n2t.net/addgene:166000 ; RRID:Addgene_166000)

  • For your References section:

    Rational Design of Novel Fluorescent Enzyme Biosensors for Direct Detection of Strigolactones. Chesterfield RJ, Whitfield JH, Pouvreau B, Cao D, Alexandrov K, Beveridge CA, Vickers CE. ACS Synth Biol. 2020 Aug 21;9(8):2107-2118. doi: 10.1021/acssynbio.0c00192. Epub 2020 Aug 11. 10.1021/acssynbio.0c00192 PubMed 32786922
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