pET11b-HA2
(Plasmid #165992)

• Purpose: Influenza HA ministem with removable HisTag
• Depositing Lab: Vesa Hytönen
• Publication: Lampinen et al J Nanobiotechnology. 2021 Jan 19;19(1):25. doi: 10.1186/s12951-021-00772-0.

Backbone

• Vector backbone: pET-11b
• Backbone size w/o insert (bp): 5676
• Total vector size (bp): 6171
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HA2
• Alt name: H1F
• Alt name: Influenza hemagglutinin ministem
• Species: Synthetic
• Insert Size (bp): 579
• Promoter: T7
• Tags / Fusion Proteins:
  • HisTag (N terminal on insert)
  • Foldon (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: BlpI (not destroyed)
• 5′ sequencing primer: none
• 3′ sequencing primer: none

Resource Information

• Supplemental Documents:
  • item2 ΔSpyCatcher-H1F_pET-11b.gbk
  • item2 ΔSpyCatcher-H1F_pET-11b.gbk
• A portion of this plasmid was derived from a plasmid made by: The gene was syntesized and cloned by Genscript.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Original publication:

Mallajosyula VV, Citron M, Ferrara F, Lu X, Callahan C, Heidecker GJ, Sarma SP, Flynn JA, Temperton NJ, Liang X, Varadarajan R. Influenza hemagglutinin stem-fragment immunogen elicits broadly neutralizing antibodies and confers heterologous protection. Proc Natl Acad Sci U S A. 2014 Jun 24;111(25):E2514-23. doi: 10.1073/pnas.1402766111. Epub 2014 Jun 9. PMID: 24927560; PMCID: PMC4078824.