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Addgene

c-myc promoter (4x TBE2 - wt)
(Plasmid #16599)

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16599 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBV-Luc
  • Backbone manufacturer
    Vogelstein Lab
  • Backbone size w/o insert (bp) 4867
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    c-myc promoter (4xTBE2 wt)
  • Alt name
    MYC
  • Species
    H. sapiens (human)
  • Entrez Gene
    MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Tcf-4 binding elements (TBEs). To construct the four tandem repeats of TBE2, we dimerized oligonucleotide cassettes containing two copies of the site and cloned the products into pBV-Luc (5'-CTAGCGGACTCTTGATCAAAGGACTCTTGATCAAAG-3' and 5'-CTAGCTTTGATCAAGAGTCCTTTGATCAAGAGTCCG-3')

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    c-myc promoter (4x TBE2 - wt) was a gift from Bert Vogelstein (Addgene plasmid # 16599 ; http://n2t.net/addgene:16599 ; RRID:Addgene_16599)
  • For your References section:

    Identification of c-MYC as a target of the APC pathway. He TC, Sparks AB, Rago C, Hermeking H, Zawel L, da Costa LT, Morin PJ, Vogelstein B, Kinzler KW. Science. 1998 Sep 4. 281(5382):1509-12. 10.1126/science.281.5382.1509 PubMed 9727977