c-myc promoter (4x TBE2 - wt)
(Plasmid #16599)

• Depositing Lab: Bert Vogelstein
• Publication: He et al Science. 1998 Sep 4. 281(5382):1509-12.

Backbone

• Vector backbone: pBV-Luc
• Backbone manufacturer: Vogelstein Lab
• Backbone size w/o insert (bp): 4867
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: c-myc promoter (4xTBE2 wt)
• Alt name: MYC
• Species: H. sapiens (human)
• Entrez Gene: MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
• Tag / Fusion Protein:
  • luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a
• 3′ sequencing primer: LucNRev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Tcf-4 binding elements (TBEs). To construct the four tandem repeats of TBE2, we dimerized oligonucleotide cassettes containing two copies of the site and cloned the products into pBV-Luc (5'-CTAGCGGACTCTTGATCAAAGGACTCTTGATCAAAG-3' and 5'-CTAGCTTTGATCAAGAGTCCTTTGATCAAGAGTCCG-3')