pFBD-SpyTag-noro-VLP
(Plasmid #165989)

• Purpose: Expresses norovirus-like particles with SpyTags presented on their surface for convenient decoration of SpyCatcher fusion proteins
• Depositing Lab: Vesa Hytönen
• Publication: Lampinen et al J Nanobiotechnology. 2021 Jan 19;19(1):25. doi: 10.1186/s12951-021-00772-0.

Backbone

• Vector backbone: pFastBac Dual
• Backbone manufacturer: Gibco
• Backbone size w/o insert (bp): 5238
• Total vector size (bp): 6825
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Gentamicin, 100 & 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SpyTag-noro-VLP
• Alt name: Noro-VP1-SpyTag
• Species: Synthetic; norovirus
• Insert Size (bp): 1674
• GenBank ID: AFV08795
• Entrez Gene: ORF2 (a.k.a. D9V40_gp2)
• Promoter: Polyhedrin
• Tag / Fusion Protein:
  • SpyTag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: -
• 3′ sequencing primer: -

Resource Information

• Supplemental Documents:
  • 17ABVGXC_2132825_pFBD-Noro-VP1SpyTag.gb
• A portion of this plasmid was derived from a plasmid made by: The gene was synthesized and subcloned by Genscript
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Original publication:
SpyCatcher:
Zakeri B, Fierer JO, Celik E, Chittock EC, Schwarz-Linek U, Moy VT, Howarth M. Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin. Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):E690-7. doi: 10.1073/pnas.1115485109. Epub 2012 Feb 24. PMID: 22366317; PMCID: PMC3311370.