pATP416-­pluxO5-­ptetO7-­crtYBI
(Plasmid #165975)

• Purpose: Expresses carotenoid biosynthesis gene in response to homoserine lactone and doxycycline in yeast expressing LuxTA and rtTA
• Depositing Lab: Jun Ishii
• Publication: Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi: 10.1038/s41467-021-22134-y.

Backbone

• Vector backbone: pRS416
• Total vector size (bp): 12296
• Vector type: Yeast Expression, Synthetic Biology
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Xanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
• Alt name: crtYB
• Species: Xanthophyllomyces dendrorhous
• Insert Size (bp): 2022
• Mutation: C66A, C1359T
• GenBank ID: AY177204.1
• Promoter: Synthetic promoter (pluxO5)

Cloning Information for Gene/Insert 1

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7, M13/pUC Forward
• 3′ sequencing primer: n/a

Gene/Insert 2

• Gene/Insert name: Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
• Alt name: CrtI
• Species: Xanthophyllomyces dendrorhous
• Insert Size (bp): 1749
• Mutation: C1281A, G1698A
• GenBank ID: AY177424.1
• Promoter: pTDH3

Cloning Information for Gene/Insert 2

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Gene/Insert 3

• Gene/Insert name: BTS1
• Alt name: Saccharomyces cerevisiae S288C farnesyltranstransferase (BTS1)
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 1008
• GenBank ID: NM_001183883.1
• Entrez Gene: BTS1 (a.k.a. YPL069C)
• Promoter: Synthetic promoter (ptetO7)

Cloning Information for Gene/Insert 3

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: n/a
• 3′ sequencing primer: M13/pUC Reverse

Resource Information

• Supplemental Documents:
  • Annotated Depositor's Sequence
• A portion of this plasmid was derived from a plasmid made by: Tet systems (pTRE-Tight)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A portion of this plasmid (the operator region of ptetO7) was derived from a plasmid (pTRE-Tight, Tet systems).