pGK415-­phlTA2-­1E
(Plasmid #165970)

• Purpose: Expresses phlTA(2-­1E) mutant
• Depositing Lab: Jun Ishii
• Publication: Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi: 10.1038/s41467-021-22134-y.

Backbone

• Vector backbone: pGK415
• Total vector size (bp): 7661
• Vector type: Yeast Expression, Synthetic Biology
• Selectable markers: LEU2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: phlF
• Species: Pseudomonas protegens
• Mutation: P5S, S6P, K86T, F109L, Q117R, E143K, codon-optimized for expression in yeast
• Promoter: pPGK1 mutant
• Tags / Fusion Proteins:
  • three copies of VP16 (VP48) (C terminal on insert)
  • Nuclear localization signal (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7, M13/pUC Forward
• 3′ sequencing primer: M13/pUC Reverse

Resource Information

• Supplemental Documents:
  • Annotated Depositor's Sequence

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry