pGK415-­rtetTAK8N, L131L
(Plasmid #165969)

• Purpose: Expresses rTetTA (K8N, L131L) mutant
• Depositing Lab: Jun Ishii
• Publication: Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi: 10.1038/s41467-021-22134-y.

Backbone

• Vector backbone: pGK415
• Total vector size (bp): 7664
• Vector type: Yeast Expression, Synthetic Biology
• Selectable markers: LEU2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: tetR
• Species: Synthetic; Escherichia coli
• Promoter: pPGK1
• Tag / Fusion Protein:
  • three copies of VP16 (VP48) (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7, M13/pUC Forward
• 3′ sequencing primer: M13/pUC Reverse

Resource Information

• Supplemental Documents:
  • Annotated Depositor's Sequence
• A portion of this plasmid was derived from a plasmid made by: Tet systems (pTet-On Advanced)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The synthetic transcription activator (rtTA) is originated from Tet systems (pTet-On Advanced, Tet systems).