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Addgene

pGK401red-­rtetTAK8N, L131L
(Plasmid #165968)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 165968 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGK401red
  • Total vector size (bp) 6713
  • Vector type
    Yeast Expression, Synthetic Biology
  • Selectable markers
    MET15

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tetR
  • Species
    Synthetic; Escherichia coli
  • Tag / Fusion Protein
    • three copies of VP16 (VP48) (C terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Tet systems (pTet-On Advanced).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The synthetic transcription activator (rtTA) is originated from Tet systems (pTet-On Advanced, Tet systems).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGK401red-­rtetTAK8N, L131L was a gift from Jun Ishii (Addgene plasmid # 165968 ; http://n2t.net/addgene:165968 ; RRID:Addgene_165968)

  • For your References section:

    Robust and flexible platform for directed evolution of yeast genetic switches. Tominaga M, Nozaki K, Umeno D, Ishii J, Kondo A. Nat Commun. 2021 Mar 23;12(1):1846. doi: 10.1038/s41467-021-22134-y. 10.1038/s41467-021-22134-y PubMed 33758180
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