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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 16596 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBV-Luc
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Backbone manufacturerVogelstein Lab
- Backbone size w/o insert (bp) 4867
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Vector typeMammalian Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namec-myc promoter (TBE 1m/2m mut)
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Alt nameMYC
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SpeciesH. sapiens (human)
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Insert Size (bp)600
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MutationTcf-4 binding elements (TBEs) mutated
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Entrez GeneMYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
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Tag
/ Fusion Protein
- luciferase (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (unknown if destroyed)
- 3′ cloning site EcoRV (unknown if destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer LucNRev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
To construct pTBE1m/2m, we used a mutated TBE1 primer (5'-CTAGCTAGCACTGGTGCATCTCCCAAACCCGGCAGCCCG-3') and a mutated TBE2 primer (5'-CTGGATATCACTGGTGCATCCCAGGGAGAGTGGAGGAAAG-3'), and subcloned the products into pBV-Luc.
See Author's Map and article for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
c-myc promoter (TBE 1m/2m - mut) was a gift from Bert Vogelstein (Addgene plasmid # 16596 ; http://n2t.net/addgene:16596 ; RRID:Addgene_16596) -
For your References section:
Identification of c-MYC as a target of the APC pathway. He TC, Sparks AB, Rago C, Hermeking H, Zawel L, da Costa LT, Morin PJ, Vogelstein B, Kinzler KW. Science. 1998 Sep 4. 281(5382):1509-12. 10.1126/science.281.5382.1509 PubMed 9727977
Map uploaded by the depositor.