PUMA Frag1-Luc
(Plasmid #16591)

• Depositing Lab: Bert Vogelstein
• Publication: Yu et al Mol Cell. 2001 Mar . 7(3):673-82.

Backbone

• Vector backbone: pBV-Luc
• Backbone manufacturer: Vogelstein Lab
• Backbone size w/o insert (bp): 4867
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PUMA promotor fragment
• Alt name: PUMA
• Species: H. sapiens (human)
• Insert Size (bp): 493
• Entrez Gene: BBC3 (a.k.a. JFY-1, JFY1, PUMA)
• Tag / Fusion Protein:
  • Luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: RVprimer3
• 3′ sequencing primer: LucNrev

Resource Information

• Articles Citing this Plasmid:
  • 10 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Contains consensus p53 binding sites BS1 and BS2, lying 230 and 144 bp upstream of the transcription start site. 5' end is 336 bp upstream of the putative transcription start site.