-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16590 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepBI-MCS-EGFP
-
Backbone manufacturerVogelstein Lab
- Backbone size w/o insert (bp) 5210
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namePUMA
-
Alt namep53 upregulated modulator of apoptosis
-
SpeciesH. sapiens (human)
-
Insert Size (bp)600
-
Entrez GeneBBC3 (a.k.a. JFY-1, JFY1, PUMA)
-
Tag
/ Fusion Protein
- 2x HA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site PvuII (destroyed during cloning)
- 3′ cloning site BglII (destroyed during cloning)
- 5′ sequencing primer na (Common Sequencing Primers)
Resource Information
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pBI-EGFP-PUMA was a gift from Bert Vogelstein (Addgene plasmid # 16590 ; http://n2t.net/addgene:16590 ; RRID:Addgene_16590) -
For your References section:
PUMA induces the rapid apoptosis of colorectal cancer cells. Yu J, Zhang L, Hwang PM, Kinzler KW, Vogelstein B. Mol Cell. 2001 Mar . 7(3):673-82. 10.1016/S1097-2765(01)00213-1 PubMed 11463391
Map uploaded by the depositor.
