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pRMCE{PuroR-5FCS-GMRWhite}-Vasa-FC31
(Plasmid #165888)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 165888 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pColE1_Omega1
  • Backbone size w/o insert (bp) 2171
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    All-in-one FC31 RMCE Cassette
  • Species
    Synthetic
  • Insert Size (bp)
    10812

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Omega1

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRMCE{PuroR-5FCS-GMRWhite}-Vasa-FC31 was a gift from Koen Venken (Addgene plasmid # 165888 ; http://n2t.net/addgene:165888 ; RRID:Addgene_165888)

  • For your References section:

    Multiplexed drug-based selection and counterselection genetic manipulations in Drosophila. Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT. Cell Rep. 2021 Sep 14;36(11):109700. doi: 10.1016/j.celrep.2021.109700. 10.1016/j.celrep.2021.109700 PubMed 34525356
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