-
Purpose(Empty Backbone)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16586 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepCEP4
-
Backbone manufacturerInvitrogen
- Backbone size (bp) 10400
-
Vector typeMammalian Expression
-
Selectable markersHygromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameNone
-
Tag
/ Fusion Protein
- 2x HA
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This construct can be used to express a double HA-tagged protein of interest, either as a C-terminal or N-terminal addition.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pHAHA was a gift from Bert Vogelstein (Addgene plasmid # 16586 ; http://n2t.net/addgene:16586 ; RRID:Addgene_16586) -
For your References section:
PUMA induces the rapid apoptosis of colorectal cancer cells. Yu J, Zhang L, Hwang PM, Kinzler KW, Vogelstein B. Mol Cell. 2001 Mar . 7(3):673-82. 10.1016/S1097-2765(01)00213-1 PubMed 11463391
Map uploaded by the depositor.
