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Addgene

pFredB-PTTG1
(Plasmid #16585)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16585 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFredB
  • Backbone size w/o insert (bp) 3939
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PTTG1
  • Alt name
    Securin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5000
  • Mutation
    3' targeting element
  • Entrez Gene
    PTTG1 (a.k.a. EAP1, ECRAR, HPTTG, PTTG, TUTR1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site See map (not destroyed)
  • 3′ cloning site See map (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

5 kb 3' targeting element, should be linearized with SalI prior to transfection.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFredB-PTTG1 was a gift from Bert Vogelstein (Addgene plasmid # 16585 ; http://n2t.net/addgene:16585 ; RRID:Addgene_16585)

  • For your References section:

    Securin is required for chromosomal stability in human cells. Jallepalli PV, Waizenegger IC, Bunz F, Langer S, Speicher MR, Peters JM, Kinzler KW, Vogelstein B, Lengauer C. Cell. 2001 May 18. 105(4):445-57. 10.1016/S0092-8674(01)00340-3 PubMed 11371342
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