pFredA
(Plasmid #16581)

• Purpose: (Empty Backbone)
• Depositing Lab: Bert Vogelstein
• Publication: Jallepalli et al Cell. 2001 May 18. 105(4):445-57.

Backbone

• Vector backbone: pFredA
• Backbone size (bp): 3838
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: See map (not destroyed)
• 3′ cloning site: See map (not destroyed)
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Targeting vector derived from pBluescript-SK. Contains SV40 promoter and 5' neomycin gene. pFredA and pFredB make up the “two-vector” targeting system developed in an effort to reduce the background rate of Geneticin-resistant colonies arising from nonhomologous integration events.