pSin-EF2-LIN28-Pur
(Plasmid #16580)

• Depositing Lab: James Thomson
• Publication: Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

Backbone

• Vector backbone: pSin4-EF2-IRES-Pur
• Backbone size w/o insert (bp): 7500
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Homo sapiens lin-28 homolog (C. elegans)
• Alt name: LIN28
• Species: H. sapiens (human)
• Insert Size (bp): 600
• GenBank ID: NM_024674
• Entrez Gene: LIN28A (a.k.a. CSDD1, LIN-28, LIN28, ZCCHC1, lin-28A)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SpeI (not destroyed)
• 5′ sequencing primer: EF-1a Forward
• 3′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: SINF-EF-G (from Dr. Robert Hawley, George Washington University) was modified to make the lentiviral backbone.
• Articles Citing this Plasmid:
  • 28 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The psPAX2 packaging plasmid and pMD2.G envelope plasmid can be used with this vector.