Ad-CBR (GFP/cAPC)
(Plasmid #16576)

• Depositing Lab: Bert Vogelstein
• Publication: Shih et al Cancer Res. 2000 Mar 15. 60(6):1671-6.

Backbone

• Vector backbone: pShuttle / pAdTrack ?
• Backbone manufacturer: Vogelstein Lab
• Vector type: Mammalian Expression, Adenoviral

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: cAPC
• Alt name: APC
• Species: H. sapiens (human)
• Insert Size (bp): 3354
• Mutation: Residues 958-2076
• Entrez Gene: APC (a.k.a. BTPS2, DESMD, DP2, DP2.5, DP3, GS, PPP1R46)
• Tag / Fusion Protein:
  • EGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ApaLI (not destroyed)
• 3′ cloning site: MluI (not destroyed)
• 5′ sequencing primer: EGFP-C

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The cAPC containing amino acids 958-2075 (nucleotides 2890–6240) was isolated from pCMV-APC by BglII digestion. This fragment was subcloned into the pEGFP-C1 (Clontech). The cassette containing the EGFP-tagged cAPC was further subcloned into the shuttle vector (pShuttle) using Apal I and Mlu I sites.