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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16574 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCI-neo
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 5500
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBeta-hCG
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Alt namebeta-chorionic gonadotropin.
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SpeciesH. sapiens (human)
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Insert Size (bp)534
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Entrez GeneCGB3 (a.k.a. CGB, CGB5, CGB7, CGB8, LHB, hCGB)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Xba1 (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCI-neo-hCG was a gift from Bert Vogelstein (Addgene plasmid # 16574 ; http://n2t.net/addgene:16574 ; RRID:Addgene_16574) -
For your References section:
Assessing tumors in living animals through measurement of urinary beta-human chorionic gonadotropin. Shih IM, Torrance C, Sokoll LJ, Chan DW, Kinzler KW, Vogelstein B. Nat Med. 2000 Jun . 6(6):711-4. 10.1038/76299 PubMed 10835692
Map uploaded by the depositor.
