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Addgene

pIVEX2.3 TEM-5
(Plasmid #16561)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16561 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIVEX2.3-MCS
  • Backbone manufacturer
    Boehringer
  • Backbone size w/o insert (bp) 3553
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TEM5
  • Alt name
    tumor endothelial marker 5
  • Species
    H. sapiens (human)
  • Mutation
    Extracellular region
  • Entrez Gene
    ADGRA2 (a.k.a. GPR124, TEM5)
  • Tag / Fusion Protein
    • 6xHis (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site SmaI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIVEX2.3 TEM-5 was a gift from Bert Vogelstein (Addgene plasmid # 16561 ; http://n2t.net/addgene:16561 ; RRID:Addgene_16561)

  • For your References section:

    Cell surface tumor endothelial markers are conserved in mice and humans. Carson-Walter EB, Watkins DN, Nanda A, Vogelstein B, Kinzler KW, St Croix B. Cancer Res. 2001 Sep 15. 61(18):6649-55. PubMed 11559528
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