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Addgene

dCas9-MSK1
(Plasmid #165601)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 165601 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    LentiCRISPR v2
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Stbl3 is also a suitable growth strain
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ribosomal protein S6 kinase A5
  • Species
    H. sapiens (human), Synthetic; S. Pyogenes
  • Insert Size (bp)
    6564
  • GenBank ID
    NM_001322227.2
  • Entrez Gene
    RPS6KA5 (a.k.a. MSK1, MSPK1, RLPK)
  • Promoter EF1a
  • Tag / Fusion Protein
    • FLAG Tag (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    dCas9-MSK1 was a gift from Isaac Hilton (Addgene plasmid # 165601 ; http://n2t.net/addgene:165601 ; RRID:Addgene_165601)

  • For your References section:

    Programmable human histone phosphorylation and gene activation using a CRISPR/Cas9-based chromatin kinase. Li J, Mahata B, Escobar M, Goell J, Wang K, Khemka P, Hilton IB. Nat Commun. 2021 Feb 9;12(1):896. doi: 10.1038/s41467-021-21188-2. 10.1038/s41467-021-21188-2 PubMed 33563994
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