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Addgene

px330 RBP1 gRNA
(Plasmid #165593)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 165593 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    px330
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RBP1
  • gRNA/shRNA sequence
    N/A
  • Species
    M. musculus (mouse)
  • Mutation
    NA
  • Entrez Gene
    Polr2a (a.k.a. 220kDa, Rpb1, Rpo2-1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    px330 RBP1 gRNA was a gift from Cornelis Murre (Addgene plasmid # 165593 ; http://n2t.net/addgene:165593 ; RRID:Addgene_165593)

  • For your References section:

    Calcium signaling instructs NIPBL recruitment at active enhancers and promoters via distinct mechanisms to reconstruct genome compartmentalization. Zhu Y, Denholtz M, Lu H, Murre C. Genes Dev. 2021 Jan 1;35(1-2):65-81. doi: 10.1101/gad.343475.120. Epub 2020 Dec 17. 10.1101/gad.343475.120 PubMed 33334824
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