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Addgene

pGL3-OF (mutant TCF-4 sites)
(Plasmid #16559)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16559 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGL3-Basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4800
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TCF-4 binding sites
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    100
  • Mutation
    3 copies of mutant Tcf-4 binding sites
  • Entrez Gene
    TCF4 (a.k.a. CDG2T, E2-2, FCD2, FECD3, ITF-2, ITF2, PTHS, SEF-2, SEF2, SEF2-1, SEF2-1A, SEF2-1B, SEF2-1D, TCF-4, bHLHb19)
  • Tag / Fusion Protein
    • Luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer RVprimer3
  • 3′ sequencing primer LucNrev
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

TCF-4 mutant binding sequence - CCTTTGGC

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-OF (mutant TCF-4 sites) was a gift from Bert Vogelstein (Addgene plasmid # 16559 ; http://n2t.net/addgene:16559 ; RRID:Addgene_16559)
  • For your References section:

    The beta-catenin binding domain of adenomatous polyposis coli is sufficient for tumor suppression. Shih IM, Yu J, He TC, Vogelstein B, Kinzler KW. Cancer Res. 2000 Mar 15. 60(6):1671-6. PubMed 10749138