pOpen-RNAse A
(Plasmid
#165565)
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PurposeRibonuclease A or RNase A; endoribonuclease purified from bovine pancreas. Important enzyme for the removal of RNA for RNA free DNA purification reactions such as plasmid DNA purification and genomic DNA purification, RNA removal from recombinant protein preparations, ribonuclease protection assays, mapping single-base mutations in DNA/RNA.
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Depositing Labs
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 165565 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepBuild
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRNAse A
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SpeciesOther
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AATG (unknown if destroyed)
- 3′ cloning site GCTT (unknown if destroyed)
- 5′ sequencing primer M13 forward
- 3′ sequencing primer M13 reverse (Common Sequencing Primers)
Terms and Licenses
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Ribonuclease A or RNase A; endoribonuclease purified from bovine pancreas. Important enzyme for the removal of RNA for RNA free DNA purification reactions such as plasmid DNA purification and genomic DNA purification, RNA removal from recombinant protein preparations, ribonuclease protection assays, mapping single-base mutations in DNA/RNA. Effectively cleaves the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide which forms a 2',3'-cyclic phosphate which is then hydrolyzed to the corresponding 3'-nucleoside phosphate.
The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information and protocols can be found at https://stanford.freegenes.org/
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pOpen-RNAse A was a gift from Drew Endy & Jennifer Molloy & FreeGenes Project (Addgene plasmid # 165565 ; http://n2t.net/addgene:165565 ; RRID:Addgene_165565)