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Addgene

CMV-CDX2-MUT
(Plasmid #16554)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16554 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHRCMV
  • Backbone manufacturer
    Vogelstein Lab
  • Backbone size w/o insert (bp) 7462
  • Vector type
    Mammalian Expression, Adenoviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CDX2
  • Species
    H. sapiens (human)
  • Mutation
    RKO mutation
  • Entrez Gene
    CDX2 (a.k.a. CDX-3, CDX2/AS, CDX3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

RKO (colorectal cancer cell line) mutation: 4 base-pair deletion (AGGCAGG to AGG) within the sequence corresponding to helix 3 of the CDX2 homeodomain.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV-CDX2-MUT was a gift from Bert Vogelstein (Addgene plasmid # 16554 ; http://n2t.net/addgene:16554 ; RRID:Addgene_16554)

  • For your References section:

    CDX2 is mutated in a colorectal cancer with normal APC/beta-catenin signaling. da Costa LT, He TC, Yu J, Sparks AB, Morin PJ, Polyak K, Laken S, Vogelstein B, Kinzler KW. Oncogene. 1999 Sep 2. 18(35):5010-4. 10.1038/sj.onc.1202872 PubMed 10490837
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