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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 16553 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHRCMV
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Backbone manufacturerVogelstein Lab
- Backbone size w/o insert (bp) 7462
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Vector typeMammalian Expression, Adenoviral
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCDX2
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SpeciesH. sapiens (human)
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Entrez GeneCDX2 (a.k.a. CDX-3, CDX2/AS, CDX3)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CMV-CDX2-WT was a gift from Bert Vogelstein (Addgene plasmid # 16553 ; http://n2t.net/addgene:16553 ; RRID:Addgene_16553) -
For your References section:
CDX2 is mutated in a colorectal cancer with normal APC/beta-catenin signaling. da Costa LT, He TC, Yu J, Sparks AB, Morin PJ, Polyak K, Laken S, Vogelstein B, Kinzler KW. Oncogene. 1999 Sep 2. 18(35):5010-4. 10.1038/sj.onc.1202872 PubMed 10490837
Map uploaded by the depositor.