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Addgene

pOpen-NotIM
(Plasmid #165518)

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Item Catalog # Description Quantity Price (USD)
Plasmid 165518 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pBuild
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    M.NotI
  • Species
    Other

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AATG (unknown if destroyed)
  • 3′ cloning site GCTT (unknown if destroyed)
  • 5′ sequencing primer M13 forward
  • 3′ sequencing primer M13 reverse
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Site-specific DNA methyltransferase; protects DNA from cleavage by NotI.

The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information and protocols can be found at https://stanford.freegenes.org/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOpen-NotIM was a gift from Drew Endy & Jennifer Molloy & FreeGenes Project (Addgene plasmid # 165518 ; http://n2t.net/addgene:165518 ; RRID:Addgene_165518)
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