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Addgene

pAAV-syn-SnFR-gamma8-minWPRE
(Plasmid #165498)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 165498 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pAAV
  • Backbone size w/o insert (bp) 3950
  • Total vector size (bp) 7130
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    iGluSnFR extracellular domain fused to TARP gamma-8 via NETO2 TM domain
  • Alt name
    cacng8
  • Species
    R. norvegicus (rat), Synthetic
  • Insert Size (bp)
    3180
  • Entrez Gene
    Cacng8
  • Promoter hSynapsin

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nhe1 (not destroyed)
  • 3′ cloning site Xba1 (not destroyed)
  • 5′ sequencing primer AGTGGGTTTTAGGACCAGG
  • 3′ sequencing primer ccacatagcgtaaaaggagca
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Susumu Tomita, Roger Nicoll, Lorin Looger

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Chimera of iGluSnFR (Addgene plasmid # 41732), Neto2 and Gamma-8
pAAV backbone is based on Addgene plasmid # 61463

Please visit https://doi.org/10.1101/2021.01.21.427382 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-syn-SnFR-gamma8-minWPRE was a gift from Andrew Plested (Addgene plasmid # 165498 ; http://n2t.net/addgene:165498 ; RRID:Addgene_165498)
  • For your References section:

    Targeted sensors for glutamatergic neurotransmission. Hao Y, Toulme E, Konig B, Rosenmund C, Plested AJR. eLife. 2023 Jan 9;12:e84029. doi: 10.7554/eLife.84029. 10.7554/eLife.84029 PubMed 36622100