TU6m-gRNAscaffHygroR
(Plasmid
#165485)
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Purpose(Empty Backbone) S. pyogenes Cas9 guide RNA expression in tilapia cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 165485 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBluescript II SK(+)
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Backbone manufacturerAgilent
- Backbone size (bp) 2961
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Vector typeCRISPR
- Promoter Oreochromis mossambicus U6
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13 Forward
- 3′ sequencing primer M13 Reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TU6m-gRNAscaffHygroR was a gift from Dietmar Kueltz (Addgene plasmid # 165485 ; http://n2t.net/addgene:165485 ; RRID:Addgene_165485) -
For your References section:
An efficient vector-based CRISPR/Cas9 system in an Oreochromis mossambicus cell line using endogenous promoters. Hamar J, Kultz D. Sci Rep. 2021 Apr 12;11(1):7854. doi: 10.1038/s41598-021-87068-3. 10.1038/s41598-021-87068-3 PubMed 33846462
Map uploaded by the depositor.
