pN23-LIC-His-FLAG
(Plasmid
#165478)
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Purpose(Empty Backbone) pET based vector for protein expression in E.coli for targets fused with N-terminal Flag and His
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 165478 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET23
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Backbone manufacturerNovagen
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Vector typeBacterial Expression
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Tag
/ Fusion Protein
- 10xHis-FLAG-TEV (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- 10xHis-FLAG-TEV (N terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Primers for LIC cloning: Upstream: add TATTTTCAATCCTACGTA to the 5' end of gene of interest. Downstream: add CCCTCAATATTATACGGG to the 3' end of GOI. Detailed cloning method available in the paper Bruni R, Kloss B. High-throughput cloning and expression of integral membrane proteins in Escherichia coli. Curr Protoc Protein Sci. 2013 Nov 5;74:29.6.1-29.6.34.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pN23-LIC-His-FLAG was a gift from Renato Bruni (Addgene plasmid # 165478 ; http://n2t.net/addgene:165478 ; RRID:Addgene_165478) -
For your References section:
High-throughput cloning and expression of integral membrane proteins in Escherichia coli. Bruni R, Kloss B. Curr Protoc Protein Sci. 2013 Nov 5;74:29.6.1-29.6.34. doi: 10.1002/0471140864.ps2906s74. 10.1002/0471140864.ps2906s74 PubMed 24510647
Map uploaded by the depositor.
