pCMV3Tag8li-hMBOAT7-3xFLAG
(Plasmid #165474)

• Purpose: Human MBOAT7 mammalian expression vector with 3 C-terminal FLAG tags.
• Depositing Lab: Martin Dorf
• Publication: Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub 2011 Sep 8.

Backbone

• Vector backbone: pCMV-3Tag-8 modified MCS
• Backbone manufacturer: Stratagene /Agilent
• Backbone size w/o insert (bp): 5200
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Membrane bound O-acyltransferase domain containing 7
• Alt name: MBOAT7
• Alt name: BC003164.1
• Species: H. sapiens (human)
• Insert Size (bp): 1416
• Entrez Gene: MBOAT7 (a.k.a. BB1, LENG4, LPIAT, LPLAT, LRC4, MBOA7, MRT57, OACT7, hMBOA-7)
• Promoter: CMV
• Tag / Fusion Protein:
  • 3xFLAG (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV, (T3)
• 3′ sequencing primer: T7, BGH polyA reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Miguel Gijon (JBC 283(44) p30235-.30245)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Backbone contains new MCS (multiple cloning site). CDS without stop codon was inserted with XhoI-NotI and it expresses in frame with 3xFLAG epitope tag. Construct insertion = Xhoi-GCC-[MBOAT7 CDS 1416 bp, no stop]-Noti-A-Clai-GTCGAG-3xFLAG-STOP