pCMV3Tag8li-hSAPS1-HA
(Plasmid #165466)

• Purpose: Human SAPS1 mammalian expression vector with C-terminal HA tag.
• Depositing Lab: Martin Dorf
• Publication: Li et al Proc Natl Acad Sci U S A. 2014 May 13;111(19):6970-5. doi: 10.1073/pnas.1322545111. Epub 2014 Apr 28.

Backbone

• Vector backbone: pCMV-3Tag-8 modified MCS
• Backbone manufacturer: Stratagene /Agilent
• Backbone size w/o insert (bp): 5200
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Protein phosphatase 6 regulatory subunit 1
• Alt name: PPP6R1, SAPS1
• Alt name: BC111980; NM_014931.3
• Species: H. sapiens (human)
• Insert Size (bp): 2643
• Entrez Gene: PPP6R1 (a.k.a. KIAA1115, PP6R1, SAP190, SAPS1)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV, (T3)
• 3′ sequencing primer: T7, BGH polyA reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Harvard Institute of Proteomics clone HsCD00341883, MGC:138185IMAGE ID 8327448.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Backbone contains new multiple cloning site and c-terminal HA epitope instead of FLAGs. Construction: Xhoi-gcc-[SAPS1 CDS 2643 bp, no stop]-Noti-a-Clai-HA epitope tag-ctt-STOP