pCMV3Tag8li-hRBM34-HA
(Plasmid #165464)

• Purpose: Human RBM34 mammalian expression vector with C-terminal HA tag.
• Depositing Lab: Martin Dorf
• Publication: Li et al Proc Natl Acad Sci U S A. 2014 May 13;111(19):6970-5. doi: 10.1073/pnas.1322545111. Epub 2014 Apr 28.

Backbone

• Vector backbone: pCMV-3Tag-8 modified MCS
• Backbone manufacturer: Stratagene /Agilent
• Backbone size w/o insert (bp): 5200
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RNA binding motif protein 34
• Alt name: RBM34
• Alt name: NM_015014.2
• Species: H. sapiens (human)
• Insert Size (bp): 1290
• Entrez Gene: RBM34
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV, (T3)
• 3′ sequencing primer: T7, BGH polyA reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Harvard Institute of Proteomics clone HsCD00332915, IMAGE ID 4809263.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Backbone contains new multiple cloning site and c-terminal HA epitope replaces FLAGs. Construction: Xhoi-acc-[RBM34 CDS 1293 bp, no stop]-Noti-a-Clai-HA tag-ctt-STOP. Uses upstream ATG initiation than in BC029451.1.